yqxD [2019-07-05 11:10:25]

unknown

locus: BSU25230

pI: 7.17

mw: 22.40 kDa

protein length: 164 aa

gene length: 495 bp

function: unknown

product: unknown

essential: no

synonyms: yqfM

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

Gene

Coordinates: 2,603,373 2,603,867

The protein

Protein family

• UPF0178 family (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|398924959D6C82918617C4B050F72D5E78EE12E9|yqxD]-[gene|F7B53E8AC92DAD15544C6208C2A5B41BABA73D6D|dnaG]-[gene|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]
  description: [Pubmed|3298998]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|3298998], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|3127379], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

  regulation

  • enzymatic activity is inhibited by (p)ppGpp during the 'stringent response

  additional information

  • An [SW|ncRNA|antisense RNA] is predicted for [gene|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA] [PubMed|20525796]

Biological materials

Mutant

• MGNA-C491 (yqxD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2489 NBRP B. subtilis, Japan]
• BKE25230 ([gene|398924959D6C82918617C4B050F72D5E78EE12E9|yqxD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAAACTCCTGCATAAAA, downstream forward: _UP4_TAAAGCATCGAATAATGTAC
• BKK25230 ([gene|398924959D6C82918617C4B050F72D5E78EE12E9|yqxD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTAAACTCCTGCATAAAA, downstream forward: _UP4_TAAAGCATCGAATAATGTAC

References

• 3298998,10411753,3127379